A healthy heart makes about two billion beats throughout life through the interaction of a 10000 protein. Researchers from the Institute of Biochemistry. Max Planck and the Technical University of Munich have determined which proteins are present in each type of heart cells. They made a "atlas" of a healthy human heart, known as cardiac proteome.
Proteins - the molecular machinery of cells that perform a number of functions. The changes taking place at the level of DNA or protein, may lead to disturbances. It is important to know which proteins are present in a healthy heart, and in what quantities.
The research team from Munich for the first time released protein hearts card in Nature Communications. Scientists have identified the protein profile of the cells in all areas of the heart. In addition, they investigated the protein composition in three different cell types: cardiac fibroblasts, smooth muscle cells and endothelial cells. Thus, they were able to display the distribution of the protein throughout the heart.
Previous studies have focused only on certain types of cells or tissues used in the affected organ. This approach has two problems. Firstly, the results did not give a complete picture of the heart in all areas and tissues, and secondly, comparative data on the healthy heart is often missing.
The researchers were surprised to find that the left and right side of the heart are similar, despite the fact that they have a completely different function.
In the next step the team wanted to check whether the data of healthy heartmonitor detection of changes in a sick body. They compared the proteomes of heart values to the data of patients with atrial fibrillation. Indeed, the results showed that the affected heart tissue was different in the proteins responsible for the supply of energy in cells.
Compare yielded another interesting conclusion: although the proteins involved in energy metabolism, have been changed in all patients, these changes differ between individuals. "These data show the importance of personalized medicine Although patients had similar symptoms from the data show that in each case was different molecular dysfunction." - says Professor Markus cranes.
However, colleagues of the Division of Cardiovascular SurgeryKran collected over 150 tissue samples. Using sophisticated cell culture techniques, they were able to extract different types of cells. A large number of heart material was the deciding factor for the study of certain areas of the heart. Scientists have made a mass spectrometric measurements.
Thanks to the clear and rapid analytical methods it is now possible to obtain data from all areas of the heart in less than two days.